一种基于大范围扫描离子电导显微镜的形貌和体积测量方法

doi:10.3969/j.issn.0372-2112.2017.05.007

摘要
图/表
参考文献(0)
相关文章 (0)

全文: PDF (4474 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要

针对现有测量方法不能同时实现大尺寸样品表面的总体和局部形貌的三维高分辨率测量以及体积计算的不足，提出了一种形貌和体积测量方法.本文首先构建了大范围扫描离子电导显微镜（Large-scale Scanning Ion Conductance Microscopy，L-SICM）系统，并利用数据拼接技术来扩展现有SICM系统的水平测量范围，从而实现样品形貌的大范围三维高分辨率测量，最后结合背景移除等数字图像处理技术来计算目标对象的体积.对亚毫米级样品的实验结果表明，基于L-SICM的测量方法可以有效完成大尺寸样品的总体和局部形貌的三维高分辨率测量及体积计算，且避免由光学测量方法引入的非线性误差.另外，采用更小的水平扫描步距（125 nm）可以减小5.82%的体积测量误差和38.12%的测量标准差，从而提高了系统测量的准确性和稳定性.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	郭仁飞
	庄健
	于德弘

关键词 ：形貌和体积测量, 扫描离子电导显微镜, 大范围, 数据拼接

Abstract：

A topography and volume measurement method is proposed to deal with the drawbacks of existing methods that they cannot simultaneously finish measuring the global and local morphologies of large-scale sample surface and volume calculation in a three-dimensional (3D) and high-resolution way.The large-scale scanning ion conductance microscopy (L-SICM) system was firstly built and the data stitching technique was used to extend the lateral measurement range of the existing SICM system,thus the large-scale,3D and high-resolution measurement result of the sample morphology can be obtained.Finally,digital image processing techniques such as subtracting background were utilized to accurately calculate the volume of the target object.Experimental results on sub-mm level sample indicate that the L-SICM based measurement method can effectively measure the global and local morphologies of large-scale sample in a 3D and high-resolution way and accomplish calculating the target volume and avoiding the nonlinear errors introduced by the optical measurement methods.Moreover,using smaller lateral scanning step (125 nm) can decrease the volumetric measurement error by 5.82% and the standard deviation of volume measurement by 38.12%,therefore improving the accuracy and stability of the measurement system.

Key words： topography and volume measurement scanning ion conductance microscopy large-scale data stitching

收稿日期: 2016-02-29

ZTFLH:	P204
	TH742

基金资助:

国家自然科学基金（No.51375363）；陕西省科技厅工业攻关项目（No.2013GY2-04）

通讯作者: 庄健 E-mail: zhuangjian@mail.xjtu.edu.cn

作者简介: 郭仁飞 男,1988年出生,博士研究生.主要研究领域为扫描离子电导显微镜的优化设计与创新应用.E-mail:guorenfei302088@163.com

引用本文:

郭仁飞, 庄健, 于德弘. 一种基于大范围扫描离子电导显微镜的形貌和体积测量方法[J]. 电子学报, 2017, 45(5): 1072-1077. GUO Ren-fei, ZHUANG Jian, YU De-hong. Topography and Volume Measurement Method Based on Large-scale Scanning Ion Conductance Microscopy. Acta Electronica Sinica, 2017, 45(5): 1072-1077.

链接本文:

http://www.ejournal.org.cn/CN/10.3969/j.issn.0372-2112.2017.05.007 或 http://www.ejournal.org.cn/CN/Y2017/V45/I5/1072

[1] Allansson L,Khatibi S,Gustavsson T,et al.Single-cell volume estimation by three-dimensional wide-field microscopy applied to astroglial primary cultures[J].Journal of Neuroscience Methods,1999,93(1):1-11.
[2] Gray J D,Kolesik P,Hoj P B,et al.Confocal measurement of the three-dimensional size and shape of plant parenchyma cells in a developing fruit tissue[J].Plant Journal,1999,19(2):229-236.
[3] Khmaladze A,Matz R L,Epstein T,et al.Cell volume changes during apoptosis monitored in real time using digital holographic microscopy[J].Journal of Structural Biology,2012,178(3):270-278.
[4] Briggman K L,Bock D D.Volume electron microscopy for neuronal circuit reconstruction[J].Current Opinion in Neurobiology,2012,22(1):154-161.
[5] Hua Y,Laserstein P,Helmstaedter M.Large-volume en-bloc staining for electron microscopy-based connectomics[J].Nature Communications,2015,Article number:6(7923).
[6] Jiao Y,Schaffer T E.Accurate height and volume measurements on soft samples with the atomic force microscope[J].Langmuir,2004,20(23):10038-10045.
[7] Schneider S W,Larmer J,Henderson R M,et al.Molecular weights of individual proteins correlate with molecular volumes measured by atomic force microscopy[J].Pflugers Archiv-European Journal of Physiology,1998,435(3):362-367.
[8] Hansma P K,Drake B,Marti O,et al.The scanning ion-conductance microscope[J].Science,1989,243(4891):641-643.
[9] Korchev Y E,Gorelik J,Lab M J,et al.Cell volume measurement using scanning ion conductance microscopy[J].Biophysical Journal,2000,78(1):451-457.
[10] Preibisch S,Saalfeld S,Tomancak P.Globally optimal stitching of tiled 3D microscopic image acquisitions[J].Bioinformatics,2009,25(11):1463-1465.
[11] Zhao Z,Zhao H,Gu F,et al.Non-null testing for aspheric surfaces using elliptical sub-aperture stitching technique[J].Optics Express,2014,22(5):5512-5521.
[12] Sternberg S R.Biomedical image processing[J].Computer,1983,1(16):22-34.
[13] Ye X,Ding Y,Duan Y,et al.Room-temperature capillary-imprint lithography for making micro-nanostructures in large areas[J].Journal of Vacuum Science & Technology B,2010,28(1):138-142.
[14] Novak P,Li C,Shevchuk A I,et al.Nanoscale live-cell imaging using hopping probe ion conductance microscopy[J].Nature Methods,2009,6(4):279-281.
[15] Ushiki T,Nakajima M,Choi M,et al.Scanning ion conductance microscopy for imaging biological samples in liquid:A comparative study with atomic force microscopy and scanning electron microscopy[J].Micron,2012,43(12):1390-1398.