Optical Imaging(OI) is a functional imaging technique based on intrinsic signals which contain abundant physiological sources.In this paper

we tried to separate artery from vena based on spectrum features of the heartbeat and respiration oscillations.Vessel network was firstly extracted from a frame of cortical image by threshold segmentation and region growing method

then we computed the spectral powers of heartbeat and respiration signals separately according to an oscillation-related physiological feature that arterial oscillation distributes between 5 Hz and 6 Hz and venous oscillation distributes between 1 Hz and 2 Hz.Separation of artery and vena was successfully achieved by utilizing the spectral power-percentage of heartbeat and respiration signals.The potential value of 0.1Hz oscillation in artery and vena separation was discussed at last.