With the completion of Human Genome draft

bioinformatic research has entered the post-genomic era.Studying of the control of gene expression and function using computational methods has gradually become the mainstream of research.For its special role in the control of eukaryotic gene expression

alternative splicing has become an important topic in genomic research.In this study

we analyzed alternative splicing of human genes based on complete coding sequences(cds) of alternatively spliced genes.As reported previously we have set up a database AsMamDB.Transcripts probably belonging to one gene were put into a single cluster.We also developed a new multi-alignment tool

ASALIGN

aiming to reveal alternative splicing patterns of transcripts belonging to a same gene and identify their alternative and non-alternative regions.Here

through filtering we chose 371 genes that have two or more different cds.Using Asalign we have identified all alternatively spliced regions in the cds of selected genes.We also studied lengths

positions of alternatively spliced regions

shifting of reading frames due to alternative splicing

as well as codon frequencies of non-alternative

alternatively spliced regions and their boundaries

and ended with some interesting results.Our study may provide important in-sights into the alternative splicing phenomenon and clues for prediction of alternative splicing.