Differential analysis is a major method to understand the process of biological evolution and the progress of diseases.Recently

differential analysis based on graph has been a hot area in system biology.Since Przulj proposed the conception of graphlet and graphlet orbits

both of them have been used in various network analyses.In this paper

we proposed two measures based on graphlet orbits

and we used them to mine within module differential co-expresssion clusters and module to module differential co-expression clusters.Applications on the data of mice for 16 months and 24 months have shown that most of the clusters are significant enrichment in some GO terms related with aging.