关键词: 下一代测序技术, RNA_seq, 选择性剪切, 剪切位点, 读段定位, 生物信息学

Abstract: With the development of sequencing technology,Next-Generation Sequencing brought opportunities and challenges to the bioinformatics.As the importance of alternative splicing in gene expression and protein diversity in eukaryotes,alternative splicing identification has been the focus of bioinformatics research.The computation methods for alternative splicing need to be improved since the appearance of Next-Generation Sequencing.We introduce the past and current research of AS firstly;then conclude and compare the research methods,software and databases of AS based on RNA-seq data;finally try to discuss the development and prospect of the computational methods on AS.

Key words: next-generation sequencing, RNA-seq, alternative splicing, splice sites, read mapping, bioinformatics